A 16S rRNA-based DNA probe and PCR method specific forListeria ivanovii
نویسندگان
چکیده
منابع مشابه
A parallel computing algorithm for 16S rRNA probe design
With the continuing rapid increase in the number of available 16S ribosomal RNA (rRNA) sequences, it is a significant computational challenge to efficiently design 16S rRNA targeted probes. In our previous work, we designed a fast software tool called ProkProbePicker (PPP) that takes O(logN) time for a worst-case scenario search. Despite this improvement, it can still take many hours for PPP to...
متن کاملPhylOPDb: a 16S rRNA oligonucleotide probe database for prokaryotic identification
In recent years, high-throughput molecular tools have led to an exponential growth of available 16S rRNA gene sequences. Incorporating such data, molecular tools based on target-probe hybridization were developed to monitor microbial communities within complex environments. Unfortunately, only a few 16S rRNA gene-targeted probe collections were described. Here, we present PhylOPDb, an online re...
متن کاملComparison of a Serological Method, a Bacteriological Method and 16S rRNA Brucella canis PCR for Canine Brucellosis Diagnosis
The objective of the study was to evaluate the sensitivity, specificity and the positive and negative predictive value of a PCR assay for canine brucellosis diagnosis using 16S rRNA specific primers compared to serology, 2 mercaptoethanol-microtiter plate agglutination tests (2ME-MPAT) and a blood culture test. A sample of 48 dogs was divided into three groups, according to the results of blood...
متن کاملQuantitative PCR with 16S rRNA-gene-targeted species-specific primers for analysis of human intestinal bifidobacteria.
A highly sensitive quantitative PCR detection method has been developed and applied to the distribution analysis of human intestinal bifidobacteria by combining real-time PCR with Bifidobacterium genus- and species-specific primers. Real-time PCR detection of serially diluted DNA extracted from cultured bifidobacteria was linear for cell counts ranging from 10(6) to 10 cells per PCR assay. It w...
متن کاملPCR primers to amplify 16S rRNA genes from cyanobacteria.
We developed and tested a set of oligonucleotide primers for the specific amplification of 16S rRNA gene segments from cyanobacteria and plastids by PCR. PCR products were recovered from all cultures of cyanobacteria and diatoms that were checked but not from other bacteria and archaea. Gene segments selectively retrieved from cyanobacteria and diatoms in unialgal but nonaxenic cultures and fro...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1993
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1993.tb05939.x